STUDIES ON BILIRUBIN SULFATE AND OTHER NONGLUCURONIDE CONJUGATES OF BILIRUBIN†

Sulfate and glucuronide conjugates of bilirubin in experimental liver injury.

Determination of the sum of bilirubin sugar conjugates in plasma by bilirubin oxidase.

BACKGROUND A reliable indicator of cholestasis is the presence of abnormal concentrations of bilirubin mono- and diglucuronide [conjugated bilirubin (CB)] in blood. A routine assay of CB is available only to those who possess a certain type of clinical analyzer. We describe a two-point manual method for CB that could be adapted as a rate assay to automated clinical analyzers. METHODS The meas...

Membrane Transporters for Bilirubin and Its Conjugates: A Systematic Review

Background: Bilirubin is a highly-hydrophobic tetrapyrrole which binds to plasma albumin. It is conjugated in the liver to glucuronic acid, and the water-soluble glucuronides are excreted in urine and bile. The membrane transporters of bilirubin diglucuronide are well-known. Still undefined are however the transporters performing the uptake of bilirubin from the blood into the liver, a process ...

Effects of thermostability of bilirubin subfractions on serum bilirubin measurement

Bilirubin is sensitive to light and oxygen. The rate of photodegradation of unconjugated bilirubin (Bu) was nearly twice that of monoconjugated bilirubin (mBc) and three fold that of diconjugated bilirubin (dBc); delta bilirubin (Bd) was the most stable against photoirradiation. In the presence of serum albumin, conjugated bilirubin (Bc: i.e., mBc and dBc) showed the highest rate of oxidation b...

Studies of bilirubin kinetics in normal adults.

This report describes studies of bilirubin kinetics in 13 healthy young adults. The plasma content of unconjugated bilirubin-(14)C was determined at frequent intervals for 24-30 hr after the intravenous injection of a tracer dose of unconjugated isotopic bilirubin. Fecal and urinary radioactivity were measured for 7 days. During this time cumulative recovery averaged 96% of the injected dose. T...